Another batch of 20 positive samples from Patrick Biancone-trained horses was last night reported to Jockey Club management as measures were put in place for pre-declaration tests on all entries from the troubled stable. All the positive samples, reported by senior racing chemist Dr David Crone, involve the same prohibited substance, the decongestant Sputolosin, which featured in the original six positives about which a Board of Inquiry opened and adjourned under the chairmanship of Jockey Club steward, David Gairns, yesterday morning. The names of the 20 horses reported positive yesterday will be known later today but the source of the ever-increasing amount of Sputolosin is still a mystery. The list of 20 horses could include a couple already on medication and some of the original six. Biancone said: 'First of all we will have to see which ones have come up as positive. 'It is something very strange and we will know more when we know the names of the horses involved. We will also know more when the feed and the bedding have been analysed. I'm sure that everything will finally be discovered but it is going to take time.' By the end of this week, Dr Crone will have finished testing all the 57 samples taken from Biancone's horses early last Thursday morning. Biancone went on: 'I just hope no more positives are found and I can start running my horses. 'The answer will be found on the racetrack. This is what I've found in my 25 years as a trainer. Always the answer to any problem you have is to be found on the racetrack.' In the meantime, the Board of Inquiry disqualified the two horses found positive to Sputolosin after racing - Biancone's impressive Class Three winner Charming Guest and his Class Four runner-up Lyphard's Lad. The other positives were detected, following tests at Biancone's insistence, prior to their intended races. The Board also introduced some interim measures to safeguard Biancone, his owners and the racing public. In a full and frank press release, the Board stated: 'Until further notice, declarations be not accepted for any horse from trainer Biancone's stable until and unless the horse concerned has passed a special pre-declaration test.' This means all Biancone's intended runners have to pass a special test just to to be permitted to be declared. They then have to pass the normal pre-race checks and tests that are run subsequent to declarations. The Board went on to order: 'All medication in Mr Biancone's stable be surrendered to the veterinary department and that any medication prescribed be kept and administered by the veterinary surgeons only, until further notice.' Gairns then adjourned the inquiry into how Charming Guest and Lyphard's Lad returned positive samples, and into any possible breaches of the Rules of Racing arising therefrom. He also adjourned the inquiry into the positive samples returned by Biancone's Lake Tahoe, Consistent Win, Highland Dancer and Deauville and, again, any possible breaches of the Rules of Racing arising from them. The adjournments resulted from the need for further investigations. These are not likely to be completed for at least a fortnight as the stipendiary stewards, all of whom sat with Gairns on the Board of Inquiry, have to watch hours and hours of video footage from Biancone's yard. Biancone added: 'The Board's decision on the medication is very good news for me, for my owners and for the punters. 'It is one less headache for me and gives protection to myself, my owners and the punters.' Biancone was also pleased by the Board of Inquiry's decision to subject all his intended runners to special pre-declaration tests. 'This gives everyone more protection if my horses are tested before they run,' he said. Biancone had expected his two runners to be disqualified in accordance with the Rules of Racing. This leaves him joint top of the standings with Ivan Allan. But Charming Guest's disqualification provides defending champion trainer John Moore with one more winner as his Rocket Green is promoted to first. This leaves Biancone and Allan on 32 winners for the season with Moore four behind. Sputolosin is the trade name for the drug dembrexine which is a prohibited substance under the Rules of Racing. It is an antibiotic which works as a decongestant on the respiratory system.